Puromycin: Effect on Messenger Rna Synthesis and Beta-galactosidase Formation in Escherichia Coli 15t.

نویسنده

  • B H SELLS
چکیده

Incubation of Escherichia coli 15T(-) in the presence of puromycin inhibits the inducible formation of beta-galactosidase to a greater extent than it inhibits protein synthesis. In E. coli 33.00 beta-galactosidase formation is also more sensitive to the presence of puromycin than is protein synthesis. In the presence of glycerol (but not in its absence) puromycin prevents the production of messenger RNA for beta-galactosidase, presumably as a result of catabolite repression.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

New polarity suppressors in Escherichia coli: suppression and messenger RNA stability.

Two new polarity suppressors (Su27 and Su78) have been isolated in Escherichia coli. Both suppress polarity in the trp and lac operons, and neither shows codon specificity for suppression. Extreme polar mutants of the lac z gene that contain either Su27 or Su78 make mRNA from the entire operon; however, no active beta-galactosidase is translated from the z-gene messenger, and the amount of dist...

متن کامل

Lifetime of bacterial messenger ribonucleic acid.

Moses, V. (University of California, Berkeley), and M. Calvin. Lifetime of bacterial messenger ribonucleic acid. J. Bacteriol. 90:1205-1217. 1965.-When cells from a stationary culture of Escherichia coli were placed in fresh medium containing inducer for beta-galactosidase, growth, as represented by increase in turbidity and by total protein synthesis, started within 30 sec. By contrast, beta-g...

متن کامل

Accumulation of untranslated lactose-specific messenger ribonucleic acid during catabolite repression in Escherichia coli.

When Escherichia coli K-12 Hfr.H was induced to synthesize beta-galactosidase in the presence of glucose, an untranslated lactose-specific mRNA (lac-mRNA), protected from decay, was found to accumulate progressively within the cells. The lac-mRNA accumulation was unaffected by the carbon source on which the cells had been grown before the induction. The amount of the lac-mRNA available for tran...

متن کامل

Regulation of beta-galactosidase synthesis in Escherichia coli by cyclic adenosine 3',5'-monophosphate.

Cyclic adenosine 3’,5’-monophosphate (cyclic AMP) increases the differential rate of synthesis of @-galactosidase in Escherichia coli made permeable by treatment with t&(hydroxymethyl)aminomethane and ethylenediaminetetraacetic acid. In normal, growing cells, cyclic AMP overcomes the transient repression of /3-galactosidase by glucose. A half-maximal effect of cyclic AMP occurs at about 7 x 10-...

متن کامل

Kinetics of the onset of catabolite repression in Escherichia coli as determined by lac messenger ribonucleic acid initiations and intracellular cyclic adenosine 3',5'-monophosphate levels.

The rates of synthesis of beta-galactosidase (EC 3.2.1.23) and the intracellular levels of cyclic 3',5'-adenosine monophosphate (cAMP) soon after the addition of glucose or glycerol to exponentially growing cultures of Escherichia coli have been determined. Within 10 s of its addition, glucose, but not glycerol, lowered the apparent initiation frequency of lac messenger ribonucleic acid. The gl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Science

دوره 148 3668  شماره 

صفحات  -

تاریخ انتشار 1965